Title:

Associate Professor

Office Information:

NCSU-CVM Research Building
RB158
1060 William Moore Rive
Raleigh, NC27607
NC State University
(919) 513-6157

Education:

Ph.D., Biological Engineering, University of Georgia B.S., Pharmaceutical Engineering, Zhejiang University

Email:

ke_cheng@ncsu.edu, ke_cheng@unc.edu

Lab Site:

https://chenglab.cvm.ncsu.edu/

Research and Publications:

Regenerative Medicine, Stem cell theory
Biomaterials, nano medicine, and stem cells for cardiovascular and pulmonary regeneration; Exosomes and microRNAs, regenerative medicine
Ke Cheng’s laboratory studies regenerative medicine by using patient-derived stem cells, biomaterials, micro-RNAs and bioengineering approaches. Translational research is a major focus of the lab. Prior to the launch of Cheng Lab at North Carolina, Dr. Cheng directed the stem cell lab for multiple human trials, including the world’s first clinical trial using cardiac stem cells to treat heart attack. Currently, we are testing the following strategies to treat cardiovascular diseases: 1) purified and more potent stem cell products derived from the natural mixture of cardiac stem cells; 2) biomaterials derived from patient's own tissues; 3) micro-RNAs involved in tissue regeneration; 4) nano theranostic agents that can augment endogenous repair; 5) novel mechanisms of cell extravasation, namely angiopellosis. Ph.D. graduate student positions are available in Prof. Cheng's lab. Applicants are expected to conduct research projects in the area of stem cell therapy and biomaterials.
Cheng K, Malliaras K, Shen D, Smith J, Houde C, Galang G, Sun B, Marbán E. Intramyocardial injection of platelet gel promotes endogenous repair and augments cardiac function in rats with myocardial infarction. Journal of the American College Cardiology. 59:245-53, 2012.
Makkar RR, Smith RR, Cheng K, Malliaras K, Thomson LE, Berman D, Czer LS, Marbán L, Mendizabal A, Johnston PV, Russell SD, Schuleri KH, Lardo AC, Gerstenblith G, Marbán E. Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial. Lancet. 379:895-904, 2012.
Lai Y, Cheng K (co-first author), Kisaalita WS. Three Dimensional Neuronal Cell Cultures More Accurately Model Voltage Gated Calcium Channel Functionality in Freshly Dissected Nerve Tissue. Plos One. 7:e45074, 2012.
Li T-S, Cheng K (co-first author), Malliaras K, Smith R, Zhang Y, Sun B, Matsushita N, Blusztajn A, Terrovitis J, Kusuoka H, Marbán L, Marbán E. Direct comparison of different stem cell types and subpopulations reveals superior paracrine potency and myocardial repair efficacy with cardiosphere-derived cells. Journal of the American College Cardiology. 59:942-53, 2012.
Malliaras K, Zhang Y, Seinfeld J, Galang G, Tseliou E, Cheng K, Sun B, Aminzadeh M, Marbán E. Cardiomyocyte proliferation and progenitor cell recruitment underlie therapeutic regeneration after myocardial infarction in the adult mouse heart. EMBO Molecular Medicine. 2012 Dec 19. doi: 10.1002/emmm.201201737.
Shen D, Cheng K (co-corresponding author), Marbán E. Dose-dependent functional benefit of human cardiosphere transplantation in mice with acute myocardial infarction. Journal of Cellular and Molecular Medicine. 16:2112-6, 2012.
Cheng K, Shen D, Smith J, Galang G, Sun B, Zhang J, Marbán E. Transplantation of platelet gel spiked with cardiosphere-derived cells boosts structural and functional benefits relative to gel transplantation alone in rats with myocardial infarction. Biomaterials. 33:2872-9, 2012.
Cheng K, Blusztajn A, Shen D, Li TS, Sun B, Galang G, Zarembinski TI, Prestwich GD, Marbán E, Smith RR, Marbán L. Functional performance of human cardiosphere-derived cells delivered in a Hyaluronan-Gelatin hydrogel. Biomaterials. 33:5317-24, 2012.
Cheng K, Shen D, Xie Y, Cingolani E, Malliaras K, Marbán E. Mechanism of extravasation of infused regenerative cells from microvessels into tissue parenchyma. Stem Cells. 30:2835-42, 2012.
Cheng K, Malliaras K, Li T-S, Sun B, Houde C, Galang G, Matsushita N, Marbán E. Magnetic enhancement of cell retention, engraftment and functional benefit after intracoronary delivery of cardiac-derived stem cells in a rat model of ischemia/reperfusion. Cell Transplantation. 21:1121-35, 2012.
Shen D, Wang X, Zhang L, Zhao X, Li J, Cheng K (co-corresponding author), Zhang J. The amelioration of cardiac dysfunction after myocardial infarction by the injection of keratin biomaterials derived from human hair. Biomaterials. 32:9290-9299, 2011.
Malliaras K, Li TS, Luthringer D, Terrovitis J, Cheng K, Chakravarty T, Galang G, Zhang Y, Schoenhoff F, Van Eyk J, Marbán L, Marbán E. Safety and efficacy of allogeneic cell therapy in infarcted rats transplanted with mismatched. Circulation. 125:100-12, 2011.
Lai Y, Asthana A, Cheng K, Kisaalita WS. Neural Cell 3D Microtissue Formation Is Marked by Cytokines’ Up-Regulation. PLoS One. 6: e26821, 2011.
Makkar R, Smith RR, Cheng K, Malliaras K, Thomson LE, Berman DS, Czer L, Marbán L, Mendizabal A, Johnston PV, Russell S, Schuleri KH, Lardo AC, Gerstenblith G, Marbán E. The CADUCEUS (CArdiosphere-Derived aUtologous Stem CElls to Reverse ventricUlar dySfunction) Trial. Circulation. 124: 2365-2374, 2011.
Cheng K, Li T-S, Malliaras K, Davis D, Zhang Y, Marbán E. Magnetic targeting enhances engraftment and functional benefit of iron-labeled cardiosphere-derived cells in myocardial infarction. Circulation Research. 106:1570-81, 2010.
Cheng K, Kisaalita WS. Exploring cellular adhesion and differentiation in a micro-/nano-hybrid polymer scaffold. Biotechnology Progress. 26:838-46, 2010.
Li T-S, Cheng K (co-first author), Lee S-T, Matsushita S, Davis D, Malliaras K, Zhang Y, Matsushita N, Smith RR, Marbán E. Cardiospheres recapitulate a niche-like microenvironment rich in stemness and cell-matrix interactions, rationalizing their enhanced functional potency for myocardial repair. Stem Cells. 28:2088-98, 2010.
Li T-S, Cheng K, Malliaras K, Matsushita N, Zhang Y, Marbán E. Expansion of human cardiac stem cells in physiological low oxygen improves cell production efficiency and potency for myocardial repair. Cardiovascular Research. 89:157-65, 2010.
Marban E, Cheng K. Heart to heart: Elusive mechanism of cell therapy. Circulation. 121:1981-4, 2010.
Zhang Y, Li T-S, Lee S-T, Wawrowsky K, Cheng K, Malliaras K, Galang G, Abraham R, Marban E. Dedifferentiation and Proliferation of Mammalian Cardiomyocytes. PLos One. 3;5(9):e12559, 2010.
Davis D, Zhang Y, Smith RR, Cheng K, Terrovitis J, Malliaras K, Li T, White A, Makkar R, Marbán E. Validation of the cardiosphere method to culture cardiac progenitor cells from myocardial tissue. PLoS One. 4:e7195, 2009.
Cheng K, Lai Y, Kisaalita WS. Three dimensional scaffolds for high throughput cell-based assay systems. Biomaterials. 29:2802-2812, 2008.
Cheng K, Lai Y, Kisaalita WS. ENStem-A human neural progenitors cultured in 3D polystyrene scaffolds for high physiological relevance. Cellutions. 3:4-6, 2008.
Lai Y, Wang L, Cheng K, Kisaalita WS. Taking Cell Culture in Drug Discovery to the Third Dimension-A Patent Review. Recent Patents on Biomedical Engineering. Vol. 1, No.2, 2008
Hu G-G, Lu X-Y, Cheng K, Liu D-Q. Application potentials of on-line near infrared spectroscopy technology in manufacturing of traditional chinese medicine. China Journal of Chinese Materia Medica. 28:1117-1119, 2003.

Tang J, Shen D, Caranasos TG, Wang Z, Allen TA, Vandergriff A, Hensley MT, Dinh PU, Cores J, Li TS, ZhangJ, Kan Q, and Cheng K. “Therapeutic microparticles functionalized with biomimetic cardiac stem cell membranes and secretome.” Nature Communications. Accepted 2016
Hensley MT, Tang J, Woodruff K, Defrancesco T, Tou S, Williams CM, Breen M, Meurs K, Keene B, Cheng K. “Intracoronary allogeneic cardiosphere-derived stem cells are safe for use in dogs with dilated cardiomyopahty.” J Cell Mol Medicine. Accepted 2016.
Allen TA, Gracieux D, Talib M, Tokarz DA, Hensley MT, Cores J, Vandergriff A, Tang J, de Andrade JB, Dinh PU, Yoder JA, Cheng K. “Angiopellosis as an Alternative Mechanism of Cell Extravasation.” Stem Cells. 2016 Jun 28. [Epub ahead of print]
Shen D, Tang J, Hensley MT, Li T, Caranasos TG, Zhang T, Zhang J, Cheng K.”Effects of Matrix Metalloproteinases on the Performance of Platelet Fibrin Gel Spiked With Cardiac Stem Cells in Heart Repair.” Stem Cells Translational Medicine. 2016 Apr 25.

Andrade JN, Tang J, Hensley MT, Vandergriff A, Cores J, Henry E, Allen TA, Caranasos TG, Wang Z, Zhang T, Zhang J, Cheng K. “Rapid and Efficient Production of Coronary Artery Ligation and Myocardial Infarction in Mice Using Surgical Clips.” PLoS One. 2015 Nov 24; 10(11):e0143221.
Vandergriff A, de Andrade JB, Tang J, Hensley MT, and Cheng K . “Intravenous administration of cardiac progenitor cell-derived exosomes mediates heart regeneration after doxorubicin-induced dilated cardiomyopathy.” Stem Cell International. 2015. Epub 2015 August 17.
Tang J, Shen D, Zhang J, Ligler FS, Cheng K. “Bispecific antibodies, nanoparticles and cells: bringing the right cells to get the job done.” Expert Opinion on Biological Therapies. 2015;15(9):1251-5
Cores J, Caranasos TG, Cheng K. “Magnetically Targeted Stem Cell Delivery for Regenerative Medicine.” Journal of Functional Biomaterials. 2015 June 6.
Henry E, Cores J, Hensley MT, Anthony S, Vandergriff A, de Andrade JBM, Allen T, Caranasos TG, Lobo LJ, Cheng K . “Adult Lung Spheroid Cells Contain Progenitor Cells and Mediate Regeneration in Rodents with Bleomycin-Induced Pulmonary Fibrosis.” Stem Cells Translational Medicine. 2015. Epub ahead of print.
Vandergriff A, Hensley TM, Cheng K . “Cryopreservation of Cardiomyocytes.” Methods in Molecular Biology. 1299:153-60. 2015.
Hensley MT, de Andrade J, Meurs K, Keene B, Tang J, Piedrahita J, Caranasos TG, Li TS, Cheng K . “Regenerative potential of canine cardiac stem cells.” Journal of Cellular and Molecular Medicine. 19(8):1805-13. 2015.
Vandergriff A, Hensley MT, Cheng K . “Cryoperservation of cardiomyocytes for cell-based assay.” Journal of Visual Experiments. 2015.
Kanazawa H, Tseliou E, Malliaras K, Yee K, Dawkins JF, De Couto G, Smith RR, Kreke M, Seinfeld J, Middleto RC, Cheng K, Luthringer D, Valle I, Chowdhury S, Fukuda K, Makkar RR, Marbán L . “Cellular post-conditioning: allogeneic cardiosphere-derived cells reduce infarct size and attenuate microvascular obstruction when administered after reperfusion in pigs with acute myocardial infarction.” Circulation Heart Failure. 8(2):322-32. 2015.

Vandergriff A, Hensley TM, Henry ET, Shen D, Anthony S, Zhang J, Cheng K . “Magnetic targeting of cardiosphere-derived stem cells with ferumoxytol nanoparticles for treating rats with myocardial infarction.” Biomaterials. 35:8528-39. 2014.
Cheng K (co-corresponding), Ibrahim A, Hensley MT, Sun B, Liu W, Smith RR, Marbán E . “CD90/Thy-1-negative cardiosphere-derived cells exhibit augmented regenerative potency in humans and mice with myocardial infarction.” Journal of the American Heart Association. 2014. In press.
Cheng K (co-corresponding), Shen D, Hensley TM, Sun D, Malliaras K, De Couto G, Middleton RC, Marbán E . “Magnetic antibody-linked nanomatchmakers for therapeutic cell targeting.” Nature Communications. 5:4880. 2014.
Xie Y, Ibrahim A, Cheng K, Malliaras K, Shen D, Sun B, Cho HC, Marbán E . “Human Cardiosphere-derived Cells Stimulate Cardiomyocyte Proliferation via β1 Integrin Signaling.” Stem Cells. 2014. Epub ahead of print.
Ibrahim A, Cheng K, Marbán E . “Exosomes as critical agents of cardiac regeneration triggered by cell therapy.” Stem Cell Reports. 2:606-19. 2014.
Aminzadeh MA, Tseliou E, Sun B, Cheng K, Malliaras K, Makkar RR, Marbán E . “Therapeutic efficacy of cardiosphere-derived cells in a transgenic mouse model of nonischemic dilated cardiomyopathy.” European Heart Journal. 2014.Epub ahead of print.
Cheng K, Malliaras K, Smith RR, Shen D Sun B, Blusztajn A, Xie Y, Ibrahim A, Aminzadeh MA, Liu W, Li TS, De Robertis MA, Marbán L, Czer LS, Trento A, Marbán E . “Human cardiosphere-derived cells from advanced heart failure patients exhibit augmented functional potency in a mouse model of myocardial infarction.” Journal of the American College of Cardiolology Heart Failure. 2:49-61. 2014.
Malliaras K, Makkar RR, Smith RR, Cheng K, Wu E, Bonow RO, Marbán L, Mendizabal A, Cingolani E, Johnston PV, Gerstenblith G, Schuleri KH, Lardo AC, Marbán E . “Intracoronary cardiosphere-derived cells after myocardial infarction: evidence of therapeutic regeneration in the final 1-year results of the CADUCEUS trial (CArdiosphere-Derived aUtologous stem CElls to reverse ventricUlar dySfunction).” Journal of the American College of Cardiolology. 63:110-22. 2014.

Malliaras K, Zhang Y, Seinfeld J, Galang G, Tseliou E, Cheng K, Sun B, Aminzadeh M, Marbán E . “Cardiomyocyte proliferation and progenitor cell recruitment underlie therapeutic regeneration after myocardial infarction in the adult mouse heart.” EMBO Molecular Medicine. 5:191-209. 2013.
Malliaras K, Smith RR, Kanazawa H, Yee K, Seinfeld J, Tseliou E, Dawkins JF, Kreke M, Cheng K, Luthringer D, Ho CS, Blusztajn A, Valle I, Chowdhury S, Makkar RR, Dharmakumar R, Li D, Marbán L, Marbán E . “Validation of contrast-enhanced MRI to monitor regenerative efficacy after cell therapy in a porcine model of convalescent myocardial infarction.” Circulation. 128:2764-75. 2013.

Makkar RR, Smith RR, Cheng K, Malliaras K, Thomson LE, Berman D, Czer LS, Marbán L, Mendizabal A, Johnston PV, Russell SD, Schuleri KH, Lardo AC, Gerstenblith G, Marbán E . “Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial.” Lancet. 379:895-904. 2012.
Cheng K, Blusztajn A, Shen D, Li TS, Sun B, Galang G, Zarembinski TI, Prestwich GD, Marbán E, Smith RR, Marbán L . “Functional performance of human cardiosphere-derived cells delivered in an in situ polymerizable hyaluronan-gelatin hydrogel.” Biomaterials. 33:5317-24. 2012.
Shen D, Cheng K (co-corresponding author), Marbán E . “Dose-dependent functional benefit of human cardiosphere transplantation in mice with acute myocardial infarction.” Journal of Cellular and Molecular Medicine. 16:2112-6. 2012.
Cheng K, Shen D, Xie Y, Cingolani E, Malliaras K, Marbán E . “Mechanism of extravasation of infused regenerative cells from microvessels into tissue parenchyma.” Stem Cells. 30:2835-42. 2012.
Lai Y, Cheng K (co-first author), Kisaalita WS . “Three Dimensional Neuronal Cell Cultures More Accurately Model Voltage Gated Calcium Channel Functionality in Freshly Dissected Nerve Tissue.” Plos One. 7:e45074. 2012.
Cheng K, Malliaras K, Shen D, Smith J, Houde C, Galang G, Sun B, Marbán E . “Intramyocardial injection of platelet gel promotes endogenous repair and augments cardiac function in rats with myocardial infarction.” Journal of the American College Cardiology. 59:245-53. 2012.
Cheng K, Shen D, Smith J, Galang G, Sun B, Zhang J, Marbán E . “Transplantation of platelet gel spiked with cardiosphere-derived cells boosts structural and functional benefits relative to gel transplantation alone in rats with myocardial infarction.” Biomaterials. 33:2872-9. 2012.
Cheng K, Blusztajn A, Shen D, Li TS, Sun B, Galang G, Zarembinski TI, Prestwich GD, Marbán E, Smith RR, Marbán L . “Functional performance of human cardiosphere-derived cells delivered in a Hyaluronan-Gelatin hydrogel.” Biomaterials. 33:5317-24. 2012.
Cheng K, Malliaras K, Li T-S, Sun B, Houde C, Galang G, Matsushita N, Marbán E . “Magnetic enhancement of cell retention, engraftment and functional benefit after intracoronary delivery of cardiac-derived stem cells in a rat model of ischemia/reperfusion.” Cell Transplantation. 21:1121-35. 2012.