Title:

Assistant Professor

Office Information:

4208D Engineering Building III
Campus Box 7115
Raleigh, NC 27695
NC State University
(919) 513-7933

Education:

Title:
Assistant Professor
Education:
B.S.ME., Purdue University, West Lafayette, IN
M.S.BME., Purdue University, West Lafayette, IN
Ph.D., University of Pittsburgh, Pittsburgh, PA
Postdoctoral Training, Colubmia University, New York, NY

Email:

dofreyte@ncsu.edu, dfreytes@unc.edu

Research and Publications:

Regenerative Medicine

Human pluripotent stem cells differentiation, Modeling inflammation and macrophage polarization, Studying interactions between tissue engineered devices and the host tissue response

Dr. Freyte’s laboratory focuses on the design and testing of bioengineered tissues mainly composed of pluripotent stem cell derived repair cells and tissue specific extracellular matrix scaffolds. The overall goal is to develop new strategies to assemble and test bioengineered constructs capable of restoring tissue function. The major goals of the laboratory are: 1) to harness the power of tissue specific extracellular matrices to guide stem cell differentiation and/or tissue remodeling in vitro and in vivo; and 2) to understand and harness the interaction between inflammatory cells and bioengineered tissue constructs in order to improve incorporation and survival.

Spiller KL, Wrona EA, Romero-Torres S, Pallotta I, Graney PL, Witherel CE, Urbanska A, Santambrogio L, Panicker LM, Feldman RA, Vunjak-Novakovic G, and Freytes DO. Differential Gene Expression in Human, Murine, and Cell Line-derived Macrophages. Experimental Cell Research. In Press (Oct 2015)

Pallotta I, Sun B, Wrona EA, and Freytes DO. BMP Protein Mediated Cross-Talk between Inflammatory Cells and Human Pluripotent Stem Cell-derived Cardiomyocytes. J Tissue Eng and Regen Med. In Press (April 2015).

Freytes DO, O’Neill JD, Duan-Arnold Y, Wrona E and Vunjak-Novakovic G. Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomycoytes. Methods in Molecular Biology. Vol 1181:69-81 2014.

Domenech M, Corrales LP, Ramirez-Vick JE, and Freytes DO. Tissue Engineering Strategies for Myocardial Regeneration. Tissue Engineering Part B: Reviews. In Press (June 2016).

Wrona EA, Peng R, Amin MR, Branski RD and Freytes DO. Extracellular matrix for vocal fold lamina propria replacement: A review. Tissue Engineering Part B: Reviews. In Press (June 2016)

Witherel CE, Graney PL, Freytes DO, Weingarten MS, and Spiller KL. Response of human macrophages to wound matrices in vitro. Wound Repair and Regen. May; 24(3):514-24 2016.

Spiller KL, Wrona EA, Romero-Torres S, Pallotta I, Graney PL, Witherel CE, Urbanska A, Santambrogio L, Panicker LM, Feldman RA, Vunjak-Novakovic G, and Freytes DO. Differential Gene Expression in Human, Murine, and Cell Line-derived Macrophages. Exp Cell Res. 2015 Oct 21. pii: S0014-4827(15)30121-X.

Reeves A, Spiller KL, Freytes DO, Vunjak-Novakovic G, and Kaplan DL. Controlled Release of Cytokines using Silk-biomaterials for Macrophage Polarization. Biomaterials, 2015 73:272-83

Wrona EA, Peng R, Born H, Amin MR, Branski RD, and Freytes DO. Derivation and Characterization of Porcine Vocal Fold Extracellular Matrix Scaffold. Laryngoscope. 2016 Apr;126(4):928-35.

Pallotta I, Sun B, Wrona EA, and Freytes DO. BMP Protein Mediated Cross-Talk between Inflammatory Cells and Human Pluripotent Stem Cell-derived Cardiomyocytes. J Tissue Eng and Regen Med. In Press (April 2015).

Johannesson B, Sui L, Freytes DO, Creusot RM, and Egli D. Towards Beta Cell Replacement for Diabetes. J EMBO Apr 1;34(7):841-55 2015.

Spiller K, Freytes DO, and Vunjak-Novakovic G. Macrophages Modulate Engineered Human Tissues for Enhanced Vascularization and Healing. Annals of Biomedical Engineering Mar;43(3):616-27 2015.

Freytes DO, O’Neill JD, Duan-Arnold Y, Wrona E and Vunjak-Novakovic G. Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomycoytes. Methods in Molecular Biology. Vol 1181:69-81 2014.

Freytes DO, O’Neill JD, Duan-Arnold Y, Wrona E and Vunjak-Novakovic G. Natural Cardiac Extracellular Matrix Hydrogels for Cultivation of Human Stem Cell-Derived Cardiomycoytes. Methods in Molecular Biology. Vol 1181:69-81 2014.

O’Neill JD, Freytes DO, Anandappa A, Oliver JA and Vunjak-Novakovic G. The Regulation of Growth and Metabolism of Kidney Stem Cells with Regional Specificity. Biomaterials, 2013 34(38):9830-41.

O’Neill JD, Anfang R, Anandappa A, Costa J, Javidfar JJ, Singh G, Freytes DO, Bacchetta MD, Vunjak-Novakovic G, and Sonett J. Decellularized Lung for Pulmonary Tissue Engineering. Annals of Thoracic Surgery 2013 96(3):1046-56.

Freytes DO, Santambrogio L, and Vunjak-Novakovic G. Optimizing Dynamic Interactions between a Cardiac Patch and Inflammatory Host Cells. Cells Tissues Organs 2011. Cells Tissues Organs, 2012 195(1-2):171-182.

Freytes DO, Kang JW, Marcos-Campos I, Vunjak-Novakovic G. Macrophage Modulate the Viability and Growth of Human Mesenchymal Stem Cells. Journal of Cellular Biochemistry, 2013 114(1):220-9.

Duan Y, Liu Z, O’Neill J, Wan LQ, Freytes DO, and Vunjak-Novakovic G. Hybrid Gel Composed of Native Heart Matrix and Collagen Induces Cardiac Differentiation of Human Embryonic Stem Cells without Supplemental Growth Factors. Journal of Cardiovascular Translational Research, 2011 4(5):605-615.

Freytes DO, Wan LQ, and Vunjak-Novakovic G. Geometry and Force Control of Cell Function. Journal of Cellular Biochemistry, 2009 108(5):1047-58. *Selected as a “Features” paper and selected for the cover.

Wainwright, J, Czajka, C, Patel, U, Freytes DO, Kimimasa, T, Gilbert T, Badylak SF. Preparation of Cardiac Extracellular Matrix from an Intact Porcine Heart. Tissue Engineering Part C Methods, 2010 16(3):525-532.

Badylak SF, Freytes DO, and Gilbert TW. Extracellular Matrix as a Biologic Scaffold Material: Structure vs. Function. Acta Biomaterialia, 2009 5(1):1-13. *Most cited article 2006-2010

Valentin JE, Freytes DO, Pesyna C, Freund J, Grasman J, and Badylak SF. Characterization of Oxygen Diffusion across a Variety of Extra-cellular Matrix Derived Scaffolds. Journal of Biomedical Materials Research Part A, 2009 91(4):1010-7.

Gilbert TW, Wognum S, Joyce EM, Freytes DO, Sacks MS, and Badylak SF. Collagen Fiber Architecture and Biaxial Mechanical Behavior of Porcine Urinary Bladder Derived Extracellular Matrix. Biomaterials, 2008 29(36):4775-82.

Reing J, Zhang L, Myers-Irvin JM, Cordero K, Freytes DO, Heber-Katz E, Badelbaeva K, McIntosh D, Dewilde A, Braunhut S, and Badylak SF. Degradation Products of Extracellular Matrix Affect Cell Migration and Proliferation, Tissue Engineering Part A 2009 15(3):605-14.

Freytes DO, Martin J, Lee AS, Velankar SS, and Badylak SF. Preparation and Rheological Characterization of a Gel form of the Porcine Urinary Bladder Matrix. Biomaterials 2008, 29(11):1630-7.

Freytes DO, Stoner R, and Badylak SF. Uniaxial and Biaxial Properties of Terminally Sterilized Porcine Urinary Bladder Matrix Scaffolds. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2008 84(2):408-14.

Stankus J, Freytes DO, Badylak SF, Wagner WR. Hybrid Nanofibrous Scaffolds from Electrospinning of a Synthetic Elastomer and Urinary Bladder Matrix. Journal of Biomaterials Science Polymer Ed, 2007 19(5):635-652.

Freytes DO, Tullius B, Stewart-Akers A, and Badylak SF. Hydrated vs. Lyophilized forms of Porcine Extracellular Matrix Derived from the Urinary Bladder. Journal of Biomedical Materials Research: Part A, 2008 87(4):862-872.

Freytes DO, Tullius R, and Badylak SF. Effect of Storage upon Material Properties of Lyophilized Porcine Extracellular Matrix Derived from the Urinary Bladder. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2006 78B:327-333.

Badylak SF, Vorp DA, Spievack AR, Simmons-Byrd A, Hanke J, Freytes DO, Thapa A, Gilbert TW, and Nieponice A. Esophageal Reconstruction with ECM and Muscle Tissue in a Dog Model. Journal of Surgical Research, 2005 128(1):87-97.

Freytes DO, Rundell AE, Vande Geest J, Vorp DA, Webster TJ, and Badylak SF. Analytically Derived Material Properties of Multilaminated Extracellular Matrix Devices using the Ball-burst Test. Biomaterials, 2005 26(27):5518-5531.

Freytes DO, Badylak SF, Webster TJ, Geddes LA, and Rundell AE. Biaxial Strength of Multilaminated Extracellular Matrix Scaffolds. Biomaterials, 2004 25(12):2353-2361.

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