Title:

Assistant Professor

Office Information:

4204B Engineering Building III
Raleigh, NC 27695
NC State University
(919) 513-8231

Education:

PhD, Bioengineering, Georgia Institute of Technology
BS, Biosystems Engineering, Clemson University

Email:

aecarso2@ncsu.edu

Lab Site:

https://awrl.bme.unc.edu

Research and Publications:

Regenerative Medicine, Pharmacoengineering
Research Interests: Materials to promote wound repair; Microgels, Synthetic platelets, Fibrin-cell interactions in wound repair, animal models of coagulopathy
Dr. Brown joined the department in August 2015. Her research centers on developing novel microgel-based materials for a variety of biomedical applications including augmentation of hemostasis, enhanced wound healing, evaluation and modulation of cellular mechanotransduction and development of biosynthetic constructs for regenerative medicine. Current projects include development of microgel-based platelet-like particles (PLPs) and other microgel assemblies for augmentation of hemostasis and regenerative medicine. Additionally, she is exploring the use of microgel-based assemblies with finely controlled elastic and viscoelastic properties for investigating and controlling cellular mechanotransduction responses.
My group’s research centers on developing novel microgel-based materials for a variety of biomedical applications including augmentation of hemostasis, enhanced wound healing, evaluation and modulation of cellular mechanotransduction and development of biosynthetic constructs for regenerative medicine. Current projects include development of microgel-based platelet-like particles (PLPs) and other microgel assemblies for augmentation of hemostasis and regenerative medicine. We are additionally exploring the use of microgel-based assemblies with finely controlled elastic and viscoelastic properties for investigating and controlling cellular mechanotransduction responses.
1. Myers, D., Qiu, Y., Fay, M., Tennenbaum, M., Chester, D., Cuadrado, J., Sakurai, Y., Baek, J., Tran, R., Ciciliano, J., Ahn, B., Mannino, R., Bunting, S., Bennett, C., Briones, M., Fernandez-Nieves, A., Smith, M., Brown, A.C., Sulcheck, T., Lam, W. Single-platelet nanomechanics measured by high-throughput cytometery. Nature Materials, 2016. doi: 10.1038/nmat4772 2. Chester, D., Brown, A.C.+, The role of biophysical properties of provisional matrix proteins in wound repair. Matrix Biology, 2016. doi: 10.1016/j.matbio.2016.08.004
3. Nandi, S., Brown, A.C.+, Platelet-mimetic strategies for modulating the wound environment and inflammatory responses. Experimental Biology and Medicine, 2016. doi: 10.1177/153570216647126 4. Brown, A.C., Hannan, R., Timmins, L., Fernandez, J., Barker, T.H., Guzzetta, N. Fibrin network changes in neonates after cardiopulmonary bypass. Anesthesiology, 2016.**Featured in AAAS Eurekalert! and Hematology Times 5. Karumbaiaha, L., Enamb, S., Brown, A.C., Saxena, T., Betancura, M., Barker, T.H., Bellamkonda, R. Chondroitin Sulfate Glycosaminoglycan Hydrogels Create Endogenous Niches for Neural Stem Cells. Bioconjugate Chemistry (epub ahead of print). 6. Brown, A.C., Dysart, M.M., Clarke, K.C., Stabenfeldt, S.E., Barker, T.H. Integrin a3b1 binding to fibronectin is dependent on the 9th type III repeat. Journal of Biological Chemistry (epub ahead of print). 7. Bachman, H.*, Brown, A.C.*+, Clarke, K.*, Dhada, K.*, Douglas, A.*, Hansen, C.*, Herman, E.*, Hyatt, J.*, Kodlekere, P.*, Saxena, S.*, Spears, S.*, Welsch, N.*, Lyon, A.L. Ultrasoft highly deformable microgels. Soft Matter, 2015, 11, 2018-28. doi: 10.1039/c5sm00047e. 8. Brown, A.C., Baker, S., Douglas, A., Keating, M., Alvarez, M., Botvinick, E., Guthold, M., Barker, T.H. Molecular interference of fibrin’s divalent polymerization mechanism enables modulation of multi-scale material properties. Biomaterials, 2015, 9, 27-36. doi: 10.1016/j.biomaterials.2015.01.010. 9. Kim, J., Park, Y., Brown, A.C., Lyon, A.L. Observation of ligand-induced dimerization with a bioresponsive hydrogel. RSC Advances, 2014, 4, 65173-65175. 10. Brown, A.C.*, Stabenfeldt, S.E.*, Ahn, B., Hannan, R., Dhada, K., Herman, E., Stefanelli, V., Guzzetta, N., Alexeev, A., Lam, W.A., Lyon, L.A., Barker, T.H. Ultrasoft microgels displaying emergent platelet-like behaviours. Nature Materials, 2014, 12, 1108-14. doi:10.1038/nmat4066. **Covered by national and international news outlets including Discover Magazine, Chemical and Engineering News, MRS 360 online, Atlanta Magazine and Times of India 11. Qui, Y.*, Brown, A.C.*, Myers, D.R., Sakurai, Y., Mannino, R., Tran, R., Ahn, B., Hardy, E., Kee, M., Kumar, S., Bao, G., Barker, T.H., Lam, W.A. Platelet mechanosensing of substrate stiffness during clot formation mediates adhesion, spreading and activation. PNAS, 2014, doi: 10.1073/pnas.1322917111. 12. Bryksin, A.V., Brown, A.C., Baksh, M.M., Finn, M.G., Barker, T.H. Learning from Nature – novel synthetic biology approaches for biomaterials design. Acta Biomater 10, 1761-1769, 2013. 13. Brown, A.C., Barker, T.H. Fibrin-based biomaterials: Modulation of macroscopic properties through rational design at the molecular level. Acta Biomater 10, 1502-1514, 2013. 14. Clarke, K.C., Douglas, A.M., Brown, A.C., Barker, T.H., Lyon, L.A. Colloid-matrix assemblies in regenerative medicine. Curr Opin Colloid Interface Sci 18, 393-405, 2013. 15. Brown, A.C., Fiore, V.F., Sulchek, T.A., Barker, T.H. Physical and chemical microenvironmental cues orthogonally control the degree and duration of fibrosis associated epithelial to mesenchymal transitions. J Pathol. 229, 25-35, 2013. 16. Markowski, M.C., Brown, A.C., Barker, T.H. Directing epithelial to mesenchymal transition through engineered microenvironments displaying orthogonal adhesive and mechanical cues. J Biomed Mater Res A, 100, 2119-27, 2012. 17. Brown, A.C., Rowe, J., Barker, T.H. Guiding epithelial cell phenotypes with engineered integrin-specific recombinant fibronectin fragments. Tissue Engineering Part A. 17, 139-50, 2011. 18. Park, E.S., Brown, A.C., DiFeo, M.A., Barker, T.H., Lu, H. Continuously-perfused, non-cross-contaminated microfluidic chamber array for massively parallel cell culture and assay. Lab Chip. 10, 571-80, 2010. **Inside front cover article 19. Carson, A.E., Barker, T.H. Engineered extracellular matrix variants for directing cell phenotype. Regen Med. 4, 593-600, 2009. *equal contributions; +corresponding author Book Chapters 1. Stabenfeldt, S.E., Brown, A.C., Barker, T.H. “Engineering ECM complexity into biomaterials for directed differentiation” in Biomaterials as Stem Cell Niches, Roy, K., Ed.; Springer-Verlag: Berlin, Germany 2010.